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KMID : 0604520120380030219
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Journal of the Society of Cosmetic Scientists of Korea
2012 Volume.38 No. 3 p.219 ~ p.224
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Effects of Dermal Cell Combination on the Formation of Basement membrane and Epidermis in Skin Equivalents
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Lee Hai-Lan
Jeong Hyo-Soon
Kim Jan-di
Yun Hye-Young
Baek Kwang-Jin
Kwon Nyoun-Soo
Min Young-Sil
Park Kyoung-Chan
Kim Dong-Seok
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Abstract
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European Union prohibited the marketing of cosmetic products containing constituents that have been examined through animal experiments. Thus, non-animal test models are needed to replace animal experiments. The reconstructed skin models are important as a test system for cosmetic, pharmaceutical, and medical device safety testing. In the present study, we tried to develop an optimal skin equivalent model containing basement membrane and epidermis. For this purpose, we used mesenchymal stem cells (MSCs) and/or preadipocytes as well as fibroblasts as the dermal matrix cells. The formation of basement membrane and epidermis was verified by immunohistochemical stains. Among various models, the epidermis was thickest when MSCs were used in the dermal matrix. Furthermore, PCNA and involucrin distribution showed that dermal matrix with MSCs resembled human skin. Therefore, skin equivalents with MSCs could be developed as a non-animal test model to replace animal experiments.
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KEYWORD
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basement membrane, mesenchymal stem cell, non-animal test, preadipocytes, skin equivalent
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